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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Adv Healthc Mater. 2021 May 29;10(13):e2001368. doi: 10.1002/adhm.202001368

Figure 5.

Figure 5.

GNR-PEG-Ce6 can kill adhered ovarian cancer spheroids via sonodynamic therapy. (A) Schematic of the process for ultrasound irradiation of adhered spheroids. (B) Representative images of live (green) and dead (red) cells in untreated, GNR-PEG-treated, and GNR-PEG-Ce6-treated SKOV-3 spheroids adhered to the LP-9 mesothelial cell layer before and after ultrasound irradiation. Scale bars indicate 400 μm. (C, D) ROS generation in SKOV-3 spheroids (C) and LP-9 mesothelial cells (D) after ultrasound irradiation (*P < 0.05). Tert-butyl hydrogen peroxide (TBHP) was used as a positive control in this assay. (E) Hematoxylin and eosin-stained (histological) cross-sections of untreated, GNR-PEG-treated, and GNR-PEG-Ce6-treated SKOV-3 spheroids. Scale bars indicate 50 μm.