Figure 6.

GNR-PEG-Ce6 can disrupt mesothelial adhesion of patient ascites spheroids and destroy them via sonodynamic therapy. (A) Representative immunofluorescence images of CA125 and CK7 expression in patient ascites spheroids. Scale bars indicate 200 μm. (B) Representative phase contrast images of untreated and GNR-PEG-Ce6-treated patient ascites spheroids. Scale bars indicate 400 μm. (C) Percentage of total patient ascites spheroids that have adhered to the LP-9 mesothelial cell layer after 72 h of incubation (*P < 0.05; ND indicates not detected). (D) ROS generation in patient ascites spheroids after 30 s of 0.8 W cm⁻² ultrasound irradiation (*P < 0.05). (E) Representative images of live (green) and dead (red) cells in untreated and GNR-PEG-Ce6 treated patient ascites spheroids that have adhered to the LP-9 mesothelial cell layer before and after ultrasound irradiation. Scale bars indicate 400 μm.