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Dissociation constants (KD) and maximum binding capacity (Bmax) of glycosylated and deglycosylated CBHI evaluated by using the Langmuir equation
| Enzyme preparation | Substrate | KD [µM] | Bmax [µmol g−1cellulose] | R2 |
|---|---|---|---|---|
| Untreated CBHI | PASC | 0.94 ± 0.17 | 8.11 ± 0.36 | 0.96 |
| MCC | 1.91 ± 0.58 | 0.28 ± 0.02 | 0.90 | |
| Deglycosylated CBHI | PASC | 1.43 ± 0.25 | 7.72 ± 0.35 | 0.96 |
| MCC | 1.40 ± 0.23 | 0.25 ± 0.01 | 0.96 |
