FIG. 8.

Expression of Flag-tagged mutant BRG1 or hBRM does not affect activation of HO-1 by arsenite or cadmium or cadmium-mediated activation of Mt I. Growth of cell lines and RNA and Western analyses were performed as described for Fig. 4. (A) Primer extension analysis of HO-1 expression. Cells were treated on day 4 with 20 μM CdCl₂ or 100 μM sodium arsenite in serum-free media for 3 h as previously described (1); primer extension analysis used primers complementary to mouse GAPDH and mouse HO-1. (C) Primer extension analysis of Mt I expression. Cells were treated on day 4 with 20 μM CdCl₂ for 8 h prior to harvesting, and primer extension analysis used primers complementary to mouse GAPDH and mouse Mt I (19). Cells were treated with cadmium chloride for 8 h instead of 24 h as was done in Fig. 5 because a time course of Cd²⁺-mediated induction of Mt I indicated that Mt I levels were slightly higher at 8 h than they were at 24 h (data not shown). (B and D) Western analysis to confirm the expression of Flag-tagged mutant BRG1 or hBRM in each sample. M, mock-treated cells; A, arsenite-treated cells; C, cadmium-treated cells.