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Northern analysis of constitutively expressed genes. RNA was isolated from cells maintained in the presence or absence of tetracycline for 4 days. Ethidium bromide staining of rRNA is shown. cDNA probes for rat β-actin, fibronectin and nucleolin, and for mouse GAPDH and major histocompatibility complex (MHC) class I were used to probe the blot for the corresponding mRNAs. Duplicate plates were extracted for Western analysis of Flag-tagged proteins as described above.
