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. 2021 Oct 23;14:5489–5500. doi: 10.2147/JIR.S315716

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© 2021 Liu et al.

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LncRNA HOTTIP was significantly upregulated in exosomes derived from the serum of CHB patients. (A) Volcano plotting of RNAseq analysis in exosomes derived from the serum of CHB patients at 48 weeks after receiving daily TAF treatment (25 mg) (Exo-serum). Exosomes derived from the serum of CHB patients before treating with TAF treatment were used as the negative control (Exo-serum-NC). (B) Applying quantitative RT-PCR analysis for verifying expressions of the top 10 upregulated lncRNAs obtained from RNAseq analysis. (C) The level of lncRNA HOTTIP in Exo-serum and Exo-serum-NC. (D) The level of lncRNA HOTTIP in HepAD38 cells treated with PBS, siRNA negative control (siRNA-NC), and siRNA against lncRNA HOTTIP (siRNA-HOTTIP). (E) The level of lncRNA HOTTIP in HepAD38 cells treated with PBS, pcDNA3.1 vector negative control (pcDNA3.1-NC), and pcDNA3.1 vector carrying lncRNA HOTTIP (pcDNA3.1-HOTTIP). (F) Cell viability of HepAD38 cells treated with siRNA negative control (siRNA-NC), pcDNA3.1 vector carrying lncRNA HOTTIP (pcDNA3.1-HOTTIP), pcDNA3.1 vector negative control (pcDNA3.1-NC), and PBS. ***p < 0.001.