Fig 4. Effect of GBCAs injection on the production of Neutrophil Elastase (NE).

Representative photomicrographs of NE immunostaining showing effects of different GBCA injections on the skin (A). Quantification number of NE expression in the dermis (B), determined by counting cells in six random microscopic fields. Injection of gadopentetate or gadodiamide increased the NE activity in the blood serum (C), and in the skin, regardless of the severity of skin lesions in both treated groups. Scale bar = 100 μm. The expression of positive cells in control mice was assigned values of 1. All data represent the mean score ± SEM. **p < 0.01.