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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Biomed Pharmacother. 2021 Sep 21;143:112172. doi: 10.1016/j.biopha.2021.112172

Figure 2.

Figure 2.

Treprostinil alleviates hepatic oxidative stress levels. (A) SOD, (B) GSH, and (C) CAT activity levels at 1-, 6-, and 24-hours post-reperfusion; (D) Lipid peroxidation levels at 48-hours post-reperfusion; (E) Antioxidants at 24-hours post-reperfusion measured by SWATH-MS based proteomics normalized to Gapdh and expressed as a fold-change over control. (F) Gclc mRNA levels at 48-hours post-reperfusion normalized to 18S. Data represented are mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 vs. control or sham; ϕP<0.05, ϕϕP<0.01, ϕϕϕP<0.001 vs. IRI-placebo (n = 3–6/group). One- or two-way ANOVA, Tukey’s multiple comparisons test. SOD: Superoxide dismutase; GSH: Glutathione; CAT: Catalase; SWATH-MS: Sequential windowed acquisition of all theoretical fragment ion mass spectra; MDA: Malondialdehyde; Gpx: Glutathione peroxidase; Gsta2: Glutathione S-transferase alpha 2; Gstm4: Glutathione S-transferase mu 4; Gclc: Glutamate-cysteine ligase catalytic subunit; CTRL: Control; PLB: IRI-placebo; TRE: IRI-treprostinil.