TABLE 1.

Abp1p shows an intermediate binding constant compared to other DNA-binding proteins

Protein	Kapp (M−1)a	Reference
Yeast Cbf1p	3 × 108 b	4
Human CENP-B	6 × 108 b	48
Xenopus 5S rRNA TFIIIA	1 × 109	25
Fission yeast Abp1p	7 × 109 b	This study
Adenovirus major late TF	1 × 1010	12
Yeast transcription factor tau	5 × 1010 b	3
Human TFIIIC	2 × 1011 b	7

^aApparent equilibrium binding constant. 

^bThe apparent equilibrium constant was determined by the electrophoretic mobility shift method (3, 7). The value was corrected for the presence of nonspecific DNA in the binding reaction, yielding a specific equilibrium binding constant.