TABLE 4.
Strain genotypeb | No. (%) of cells
|
||||||
---|---|---|---|---|---|---|---|
Normal | Unequal | Lagging | Placement | Asymmetric | “Cut” | Total | |
WT | 183 (100) | 0 (<0.6) | 0 (<0.6) | 0 (<0.6) | 0 (<0.6) | 0 (<0.6) | 183 |
Δcbh1 | 123 (100) | 0 (<0.8) | 0 (<0.8) | 0 (<0.8) | 0 (<0.8) | 0 (<0.8) | 124 |
Δabp1 | 147 (92.5) | 6 (3.8) | 3 (1.9) | 3 (1.9) | 0 (<0.6) | 0 (<0.6) | 159 |
Δabp1 Δcbh1 | 71 (69.6) | 14 (13.7) | 6 (5.9) | 6 (5.9) | 3 (2.9) | 2 (2.0) | 104 |
Propidium iodide-stained dividing cells were scored for the shown patterns. Cells with a placement defect contained one chromatin mass at the midpoint of the cell and the other mass at one end of the cell. Cells exhibiting asymmetric chromatin distribution or a “cut” phenotype contained a septum. Only a fraction of the cells in other categories had formed a septum.
See footnote a to Table 3 for strain names. WT, wild type.