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Reverse transcription reaction system.
| Composition | Volume (µl) | Final concentration |
|---|---|---|
| DNAse treated RNA | 4 | — |
| 5 × buffer | 4 | 1 |
| dNTPs (10 mM) | 1 | 0.5 mM |
| Oligo (dT)18 (50 μM) | 1 | 25 pM |
| Random primer (100 μM) | 0.5 | 25 pM |
| MMLV reverse transcriptase (200 U/μl) | 1 | 10 U/µl |
| DEPC H2O | 8.5 | — |
| Total volume | 20 | — |
