Table 4.

Reaction conditions.

NR2B				GAPDH
Experimental steps	Temperature	Time	Number of cycles	Temperature	Time	Number of cycles
Predenaturation	94°C	2 min	35	94°C	2 min	35
Transsexual	94°C	30 sec		94°C	30 sec
Annealing	52°C	30 sec		55°C	30 sec
Extend	72°C	30 sec		72°C	30 sec
Extend	72°C	10 min		72°C	10 min