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HT22 cells were treated with glutamate and E-64d for 24 h. The protein expression of Beclin-1 (a), Caspase-3 (b), LC3-II/I (c), Cytc (d), Drp1 (e), Hsc70 (f), and Lamp2a (g) was measured by Western blotting. Three independent experiments were performed, and the data are expressed as the mean ± SD. ∗∗P < 0.01 vs. the Control group. ##P < 0.01 vs. the Glutamate group. n.s.: not significant.
