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. 2019 Nov 26;26(8):3956–3969. doi: 10.1038/s41380-019-0606-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Lnx1 deletion decreases CA3 neuronal reaction to social recognition and synaptic activity. a Schematic of fibre recording experimental set-up and localization of optical fibre (red dots) in CA3 area. b Quantification of area under ΔF/F curve of empty, stranger, or littermate interaction in PW3 WT and Lnx1^−/− mice. n = 5 for WT or Lnx1^−/− mice interact with empty or stranger and n = 8 mice for littermate. c Representative traces of bulk fluorescence signal from hippocampal CA3 neurons, with black areas indicating interaction bouts. Black line for WT mice and red line for Lnx1^−/− mice. d Heat maps (left) showing the ΔF/F in response to the first interaction bout for two-group mice. Time course (right) of average GCaMP6s event-locked to interaction with littermate for the 1st interaction bout, n = 8 mice for per group. e Quantification of area under ΔF/F curve of 1st, 2nd, and 3rd interaction bout. n = 7 mice for per group. f Interaction bouts of investigation with littermate for WT mice and Lnx1^−/− mice. n = 7 mice for per group. g Representative traces of mEPSC recorded (upper), Scale bar: 20 pA (vertical) × 0.5 s (horizontal). Summary of cumulative probability of mEPSC amplitudes and frequency in CA3 (middle) and CA1 (bottom) pyramidal neurons. Insets show average mEPSC amplitudes and frequency. n = 16 neurons from five WT mice and n = 21 neurons from five Lnx1^−/− mice for CA3 area, n = 13 neurons from three WT mice and n = 15 neurons from four Lnx1^−/− mice for CA1 area. h Representative traces of AMPAR EPSCs recorded at −70 mV and NMDAR EPSC at +40 mV. Scale bar: 100 pA (vertical) × 20 ms (horizontal). Ratio of NMDAR/AMPAR in CA3 and CA1 were quantified respectively for per group. n = 16 neurons from three WT mice and n = 14 neurons from five Lnx1^−/− mice for CA3 area, n = 9 neurons from three WT mice and n = 9 neurons from three Lnx1^−/− mice for CA1 area. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; two-way ANOVA with Turkey’s multiple comparison post hoc test (b, e) and unpaired t-test (f, g, h)