Figure 2. Current amebicidal screening techniques.

(A) Current high-throughput amebicidal drug screening techniques incubate trophozoites in multiwell screening plates with compounds of interest. (B) The screening plates are then assayed through Alamar Blue or CellTiter-Glo cell viability assays to determine cell viability as a function of drug concentration. (C) Compounds with potent activity at low drug concentrations are considered hits and assessed further to determine the IC₅₀ through secondary screens.