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. Author manuscript; available in PMC: 2022 Oct 25.
Published in final edited form as: Curr Biol. 2021 Aug 23;31(20):4486–4498.e6. doi: 10.1016/j.cub.2021.07.081

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Bacterial and virus strains
Biological samples
Chemicals, peptides, and recombinant proteins
Uridine Sigma-Aldrich Cat# U3750
Uracil Sigma-Aldrich Cat# U0750
Benomyl Sigma-Aldrich Cat# 45339
Trolox Sigma-Aldrich Cat# 238813
Paclitaxel (Taxol) Cytoskeleton, Inc Cat# TXD01
β-casein Sigma-Aldrich Cat# C6905
Catalase Sigma-Aldrich Cat# C9322
Glucose oxidase Sigma-Aldrich Cat# G2133
Tubulin Cytoskeleton, Inc Cat# T238P
X-rhodamine-labeled tubulin Cytoskeleton, Inc Cat# TL620M
(3-Aminopropyl)triethoxysilane (APTES) Sigma-Aldrich Cat# A3648
Glutaraldehyde Sigma-Aldrich Cat# G6257
D-(+)-Glucose Sigma-Aldrich Cat# G7528
GTP Cytoskeleton, Inc Cat# BST06
ATP Cytoskeleton, Inc Cat# BSA04
Critical commercial assays
DNeasy Plant Mini Kit QIAGEN Cat# 69104
QIAquick Gel Extraction Kit QIAGEN Cat# 28706
Deposited data
Experimental models: Cell lines
Experimental models: Organisms/strains
A. nidulans: JZ383: ΔnudF::pyr4; GFP-nudAHC; argB2::[argB*-alcAp::mCherry-RabA] J. Zhang (Uniformed Services University) [35] N/A
A. nidulans: LZ36: GFP-nudAR3086C; ΔnkuA::argB; pyroA4 L. Zhuang (Uniformed Services University) [53] N/A
A. nidulans: RQ2: GFP-nudAHC; argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyrG89; pyroA4; yA2 R. Qiu (Uniformed Services University) [62] N/A
A. nidulans: RQ8: GFP-nudAF208V; argB2::[argB*-alcAp::mCherry-RabA]; pyrG89; wA2 R. Qiu (Uniformed Services University) [62] N/A
A. nidulans: RQ165: nudF-GFP-AfpyrG; argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyroA4; pyrG89; wA2 R. Qiu (Uniformed Services University) [35] N/A
A. nidulans: RQ275: nudF6; gpdA-ΔC-hookA-S-AfpyrG; GFP-nudAHC; pyrG89; yA2; possibly ΔnkuA::argB R. Qiu (Uniformed Services University) [35] N/A
A. nidulans: RQ287: GFP-nudAHC; gpdA-ΔC-hookA-S-AfpyrG; yA2 R. Qiu (Uniformed Services University) [35] N/A
A. nidulans: XX60: ΔnudA::pyrG; pyrG89 X. Xiang (Uniformed Services University) [56] N/A
A. nidulans: XX222: GFP-nudAHC; argB2::[argB*-alcAp::mCherry-RabA]; yA2; pantoB100 X. Xiang (Uniformed Services University) [7] N/A
A. nidulans: XX514: GFP-nudAHC; ΔyA::NLS-DsRed; possibly ΔnkuA::argB X. Xiang (Uniformed Services University) [35] N/A
A. nidulans: XX566: nudF-GFP-AfpyrG; gpdA-ΔC-hookA-S-AfpyrG; argB2::[argB*-alcAp::mCherry-RabA]; wA2 X. Xiang (Uniformed Services University) [35] N/A
A. nidulans: XX634: ΔnudF::pyr4; GFP-nudAR1602E, K1645E; argB2::[argB*-alcAp::mCherry-RabA]; possibly ΔnkuA::argB X. Xiang (Uniformed Services University) [35] N/A
A. nidulans: XY13: p150-GFP-AfpyrG; ΔnkuA::argB; pyrG89; pyroA4 X. Yao (Uniformed Services University) [7] N/A
A. nidulans: XY42: argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyrG89; yA2; pantoB100 X. Yao (Uniformed Services University) [79] N/A
A. nidulans: JZ504: GFP-nudAHC; ΔhookA-AfpyrG; argB2::[argB*-alcAp::mCherry-RabA]; pyroA4 This paper N/A
A. nidulans: RQ102: GFP-nudAHC; ΔhookA-AfpyrG; argB2::[argB*-alcAp::mCherry-RabA]; pantoB100; yA2 This paper N/A
A. nidulans: RQ293: nudF6; gpdA-ΔC-hookA-S-AfpyrG; GFP-nudAR3086C This paper N/A
A. nidulans: RQ295: gpdA-ΔC-hookA-S-AfpyrG; GFP-nudAR3086C This paper N/A
A. nidulans: RQ300: GFP-nudAK2599A(wA-AAA3); argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyroA4; yA2 This paper N/A
A. nidulans: RQ301: GFP-nudAE2663Q(wB-AAA3); argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyroA4; yA2 This paper N/A
A. nidulans: RQ306: ΔnudF::pyr4; GFP-nudAK2599A(wA-AAA3); argB2::[argB*-alcAp::mCherry-RabA] This paper N/A
A. nidulans: RQ307: ΔnudF::pyr4; GFP-nudAE2663Q(wB-AAA3); argB2::[argB*-alcAp::mCherry-RabA] This paper N/A
A. nidulans: RQ309: GFP-nudAK2599A(wA-AAA3); gpdA-ΔC-hookA-S-AfpyrG This paper N/A
A. nidulans: RQ311: GFP-nudAK2599A(wA-AAA3); ΔyA::NLS-DsRed; possibly ΔnkuA::argB This paper N/A
A. nidulans: RQ312: GFP-nudAE2663Q(wB-AAA3); gpdA-ΔC-hookA-S-AfpyrG This paper N/A
A. nidulans: RQ314: GFP-nudAE2663Q(wB-AAA3); ΔyA::NLS-DsRed; possibly ΔnkuA::argB This paper N/A
A. nidulans: RQ317: GFP-nudAK2599A(wA-AAA3); gpdA-ΔC-hookA-S-AfpyrG; argB2::[argB*-alcAp::mCherry-RabA] This paper N/A
A. nidulans: RQ318: GFP-nudAK2599A(wA-AAA3); gpdA-ΔC-hookA-S-AfpyrG; nudF6 This paper N/A
A. nidulans: RQ323: nudAK2599A(wA-AAA3); p150-GFP-AfpyrG; ΔnkuA::argB; argB2::[argB*-alcAp::mCherry-RabA]; pantoB100; yA2 This paper N/A
A. nidulans: RQ324: nudAE2663Q(wB-AAA3); p150-GFP-AfpyrG; ΔnkuA::argB; argB2::[argB*-alcAp::mCherry-RabA]; pantoB100; yA2 This paper N/A
A. nidulans: RQ328: GFP-nudAE2663Q(wB-AAA3); argB2::[argB*-alcAp::mCherry-RabA]; ΔhookA-AfpyrG This paper N/A
A. nidulans: RQ329: GFP-nudAK2599A(wA-AAA3); argB2::[argB*-alcAp::mCherry-RabA]; ΔhookA-AfpyrG This paper N/A
A. nidulans: RQ330: GFP-nudAK2599A(wA-AAA3); argB2::[argB*-alcAp::mCherry-RabA] This paper N/A
A. nidulans: RQ331: GFP-nudAE2663Q(wB-AAA3); argB2::[argB*-alcAp::mCherry-RabA] This paper N/A
A. nidulans: RQ332: GFP-nudAK2599A(wA-AAA3); alcAp-nudKArp1-pyr4; possibly argB2::[argB*-alcAp::mCherry-RabA]; possibly p25-S This paper N/A
A. nidulans: RQ333: GFP-nudAE2663Q(wB-AAA3); alcAp-nudKArp1-pyr4; possibly argB2::[argB*-alcAp::mCherry-RabA]; possibly p25-S This paper N/A
A. nidulans: RQ338: GFP-nudAF208V; argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyrG89; pyroA4 This paper N/A
A. nidulans: RQ343: GFP-nudAK2599A, F208V; argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyroA4 This paper N/A
A. nidulans: RQ344: GFP-nudAE2663Q, F208V; argB2::[argB*-alcAp::mCherry-RabA]; ΔnkuA::argB; pyroA4 This paper N/A
A. nidulans: RQ354: nudF-GFP-AfpyrG; nudAK2599A(wA-AAA3); argB2::[argB*-alcAp::mCherry-RabA]; possibly ΔnkuA::argB; pyrG89 This paper N/A
A. nidulans: RQ355: nudF-GFP-AfpyrG; nudAE2663Q(wB-AAA3); argB2::[argB*-alcAp::mCherry-RabA]; possibly ΔnkuA::argB; pyrG89 This paper N/A
A. nidulans: XX632: GFP-nudAR3086C; ΔnudF::pyr4; argB2::[argB*-alcAp::mCherry-RabA]; possibly ΔnkuA::argB; possibly pyrG89 This paper N/A
A. nidulans: XX644: GFP-nudAK2599A(wA-AAA3); nudF6; argB2::[argB*-alcAp::mCherry-RabA]; possibly ΔnkuA::argB This paper N/A
A. nidulans: XX684: nudF-GFP-AfpyrG; nudAK2599A(wA-AAA3); gpdA-ΔC-hookA-S; possibly ΔnkuA::argB; possibly pyrG89; possibly argB2::[argB⁄-alcAp::mCherry-RabA] This paper N/A
A. nidulans: XX689: nudF-GFP-AfpyrG; nudAE2663Q(wB-AAA3); gpdA-ΔC-hookA-S; possibly ΔnkuA::argB; possibly pyrG89; possibly argB2::[argB⁄-alcAp::mCherry-RabA] This paper N/A
A. nidulans: XX725: nudA1; ΔyA::NLS-DsRed This paper N/A
Oligonucleotides
Primer: WAF: TTCTGGTGCAACCATGACACTGTTTGCCG This paper N/A
Primer: WAR:
GTGTCATGGTTGC ACCAGAACCGGGAGGAC		 This paper N/A
Primer: NudA58:
AGAAAGATCGTGGACAACCTC		 L. Zhuang (Uniformed Services University) [53] N/A
Primer: NdA310
CACGGTTAACTCGTTCGTATG		 L. Zhuang (Uniformed Services University) [53] N/A
Primer: WBF:
ATCTTCTGCGATCAAATCAACCTGCCGGCTC		 This paper N/A
Primer: WBR:
CAGGTTGATTTG ATCGCAGAAGATAACCAGCCAA		 This paper N/A
Primer: ORF-F2:
GTCCTTTCAAGCAAGCAGAGAATG		 J. Zhang (Uniformed Services University) [7] N/A
Primer: UTR-R2:
GATGCTGAGCTTGCTGCCTG		 J. Zhang (Uniformed Services University) [7] N/A
Recombinant DNA
Software and algorithms
FIJI/ImageJ NIH, USA https://imagej.net/Fiji
Kymograph and velocity (FIJI plugin) EMBL https://www.embl.de/eamnet/html/kymograph.html
IPLab software Scanalytics, Inc https://www.bioprocessonline.com/doc/image-processing-software-0001
Prism 8 GraphPad https://www.graphpad.com/scientific-software/prism/
Other
8-well #1 chambered coverglass with cover Thermo Fisher Cat# 155411