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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Cell Biochem Biophys. 2021 Jul 15;79(3):659–668. doi: 10.1007/s12013-021-01016-6

Figure 4. Complexity of human stratum corneum ceramides.

Figure 4.

(A) Product ions of the m/z 678.5 demonstrate that C18- and C20-sphingosines and phytosphingosines are the major sphingoid bases in ceramides with the molecular and pseudomolecular ion of the m/z 678.5. This corresponds to structural formulas CER(N26:0)S(18), CER(N24:0)S(20), CER(N26:0)P(18), and CER(N24:0)P(20). Inserts show the nomenclature of MS/MS fragments (left) and the presence of sphingoid bases with odd chain length (C19) (right) within ceramides with the m/z 678.5. (B) Precursor ions scan for the m/z 264.4 demonstrates the presence and the abundance of major NS and AS ceramide as wells as major EOS ceramide species that contain S(18) sphingoid base. Both experiments were performed with declustering potential of 80V and collision energy of 40eV on AB Sciex 6500QTRAP instrument.