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. 2021 Oct 6;24(11):103223. doi: 10.1016/j.isci.2021.103223

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© 2021 The Authors.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Knockdown of STK39 induces CCA cells apoptosis and cell-cycle arrest

QBC-939 and RBE cells were transfected with STK39 shRNA (sh2) or control shRNA (sh NC); HUCCT1 cells were infected with STK39 overexpression lentivirus (OE) or control lentivirus (NC).

(A) Cell cycle analysis of STK39 stable knockdown or overexpression cells.

(B) The percentage of apoptotic cells of STK39 stable knockdown or overexpression cells was analyzed by Annexin V-FITC/PI staining assay. ∗p<0.05; ∗∗p<0.01; ∗∗∗p<0.001. Data were shown as mean ± SEM.