TABLE 4.
Genotyping of S. aureus strains isolated in nasal carriers in 1988 and 1995
| Carrier no. | Carrier indexa | Yr of culture | Isolation of S. aureus | Genotyping
|
|||
|---|---|---|---|---|---|---|---|
| RAPD | PFGE | No. of protein A repeatsb | Coagulase gene | ||||
| 1 | 0.80 | 1988 | Yes | NDc | ND | ND | ND |
| 1995 | No | ||||||
| 2 | 0.83 | 1988 | Yes | AAA | A | 3 | A |
| 1995 | Yes | BBB | B | 9 | B | ||
| CBB | C | 13 | C | ||||
| 3 | 0.83 | 1988 | Yes | ND | ND | ND | ND |
| 1995 | No | ||||||
| 4 | 0.83 | 1988 | Yes | DCC | D | 10d | D |
| 1995 | Yes | EAA | E | 3 | A | ||
| BBD | B | 10d | B | ||||
| 5 | 0.83 | 1988 | Yes | BBB | B | 10d | B |
| 1995 | Yes | BBD | F | 11 | E | ||
| BBD | G | 11 | E | ||||
| BBD | F | 10d | E | ||||
| 6 | 0.83 | 1988 | Yes | FDEd | H | 12 | F |
| 1995 | Yes | FDEd | I | 7 | B | ||
| 7 | 0.83 | 1988 | Yes | ND | ND | ND | ND |
| 1995 | No | ||||||
| 8 | 0.92 | 1988 | Yes | ND | ND | ND | ND |
| 1995 | No | ||||||
| 9 | 0.92 | 1988 | Yes | AAA | J | 1 | A |
| 1995 | Yes | FDF | G | 11 | E | ||
| 10 | 0.92 | 1988 | Yes | ND | ND | ND | ND |
| 1995 | No | ||||||
| 11 | 1.00 | 1988 | Yes | AAA | A | 9 | A |
| 1995 | Yes | GDE | Ge | 13 | E | ||
| GDE | Ke | 13 | E | ||||
| 12 | 1.00 | 1988 | Yes | HCCd | Ld | 9 | Ed |
| 1995 | Yes | HCCd | Ld | 5 | Ed | ||
| ICC | M | 5 | Ed | ||||
| 13 | 1.00 | 1988 | Yes | AEAd | Ad | 7d | Ad |
| 1995 | Yes | AEAd | Ad,f | 7d | Ad | ||
| AEAd | Nf | 7d | Ad | ||||
| 14 | 1.00 | 1988 | Yes | DCCd | Od | 9d | Gd |
| 1995 | Yes | DCCd | Od | 9d | Gd | ||
| 15 | 1.00 | 1988 | Yes | HCC | D | 10d | Ed |
| 1995 | Yes | JBB | B | 10d | Ed | ||
| 16 | 1.00 | 1988 | Yes | GCG | C | 10 | E |
| 1995 | Yes | FBE | P | 9 | F | ||
| 17 | 1.00 | 1988 | Yes | FDF | G | 8d | H |
| 1995 | Yes | BBB | B | 8d | B | ||
| Total no. of genotypes | 15 | 14 | 10 | 8 | |||
The carrier index is defined as the proportion of nasal cultures yielding S. aureus for a given individual.
The number of protein A repeats as assessed by RFLP analysis.
ND, not done.
Long-term persistence of molecular type.
Closely related genotypes; only a two-band difference.
Closely related genotypes; only a three-band difference.