Fig. 2.

General scheme for rapid pulse voltammetry-partial least squares regression (RPV-PLSR). a Dopamine neurons in the substantia nigra and ventral tegmental area (SN/VTA) of DAT^IREScre mice were transfected with the excitatory opsin Chrimson. Basal and optically stimulated dopamine and serotonin levels were recorded from the striatum (STR) using the alternating RPV-fast-scan cyclic voltammetry waveform (Fig. 1c). b Electrodes used for in vivo measurements were then post-calibrated to provide data to build a PLSR model for analyte identification and quantification. c The in vivo data were analyzed using the model