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. 2021 Oct 27;10(10):72. doi: 10.1038/s41389-021-00361-8

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Heatmap showing the molecular subtype marker expression in proneural GSCs (PN12, PN16, and PN19), and mesenchymal GSCs (ME23, ME27, and ME29) with or without TGF-β1 treatment for 4 or 8 h. Proneural markers: DLL3, OLIG2, ASCL1, CD133, and SOX2. Mesenchymal markers: CD44, CHI3L1, TIMP1, BCL3, COL3A1, MGP, and POSTN. Z-scores were calculated from the △Ct values obtained in the quantitative polymerase chain reaction analysis. B Western blotting analysis of SMAD2, p-SMAD2, SMAD3, and p-SMAD3 expression in proneural GSCs (PN12, PN16, and PN19) and mesenchymal GSCs (ME23, ME27, and ME29) with or without TGF-β1 treatment for 8 h. C Kaplan–Meier survival curves for patients with mesenchymal GBM and a high Smad3 gene signature (n = 44) and those with a low Smad3 gene signature (n = 33). D Protein–protein interaction networks indicated by STRING and Cytoscape software show overlapping peaks in both proneural and mesenchymal GSCs and also identify those that are unique to a particular subtype of GSCs or shared between the proneural and mesenchymal GSCs. E Lysates from proneural GSCs and mesenchymal GSCs were subjected to immunoprecipitation using anti-Smad3 antibody and then immunoblotted with anti-E2F4 and anti-EP300 antibodies. GSCs glioblastoma stem cells.