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. 2021 Oct 27;7:315. doi: 10.1038/s41420-021-00703-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Negative control or siRNA (si-RBM15#1 and #2) was transfected into HCC-LM3 (a) and MHCC97H (b), respectively. The efficiency of knockdown was tested by western blotting and the proliferation capacities of HCC cells were detected by CCK-8 and colony-formation assays (***p < 0.001, ****p < 0.0001; two-way ANOVA and t test); c, d EdU assays were applied to compare the proliferation abilities of HCC-LM3 (c) and MHCC97H cells (d) (scale bar, 200 μm); bar charts showed the percentage of cells in S phage based on the results of EdU assays (**p < 0.01; t test); e, f wound-healing assays were performed to compared the migration capabilities of HCC-LM3 (e) and MHCC97H (f) cells (scale bars, 200 μm); the percentage of healed area were quantified by bar charts (****p < 0.0001; t test); g, h transwell assays were applied to detect the migration and invasion abilities of HCC-LM3 (g) and MHCC97H (h) cells after silencing RBM15 (scale bars, 200 μm); bar charts showed the relative count of two groups of HCC cells, which passed through the chamber membranes when referred to negative control groups (****p < 0.0001; t test); The data are presented as mean ± SD.