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. 2021 Oct 27;11:21224. doi: 10.1038/s41598-021-00627-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of mTORC1 signal-related factors after intramuscular injection of MSCs. Protein expression of phosphorylated Akt (Ser473, A), Akt (B), phosphorylated p70S6K (Thr389, C), p70S6K (D), phosphorylated rpS6 (Ser240/244, E), rpS6 (F), phosphorylated 4EBP1 (Thr37/46, G), 4EBP1 gamma form ratio (H), and representative bands (I). Data are expressed relative to the control leg at 2 days post intramuscular injection of MSCs as the mean ± SE. Tendency or significant main effects of MSC were observed for phosphorylated Akt, Akt, phosphorylated p70S6K, p70S6K, phosphorylated rpS6, rpS6, and phosphorylated 4EBP1 (P = 0.0581, P < 0.0001, P < 0.001, P < 0.01, P < 0.0001, P < 0.001, and P < 0.001, respectively). A significant main effect of time was observed for phosphorylated p70S6K (P < 0.05).