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. 2021 Oct 27;12:6201. doi: 10.1038/s41467-021-26431-4

Fig. 3. Preventive and therapeutic effect of CR but not caloric cycling in reducing metastatic burden.

Fig. 3

a Representative images demonstrating the appearance of white masses in india ink-stained lungs, indicative of metastases. b Macroscopic quantification of lung metastases. c Macroscopically, lung metastases were scored in a blinded fashion and divided into three groups based on size: 0.5 < 1 mm, 1 < 1.15 mm, and >1.5 mm. Large primary tumors have a greater metastatic index. d Representative images and histological quantification of lung metastatic areas [H&E staining, original magnification ×200]. Scale bar = 200 μm. The lung and primary tumors presented in Fig. 2 and Supplementary Fig. 3 for each dietary intervention are from the same mouse. Most of the data are represented as scatter plots with mean values ± SEM. One-way ANOVA with Tukey post hoc analysis was used to determine statistical significance with *p < 0.05 compared to AL. b, c AL, n = 11; FMD post-4T1, n = 12; LCC post-4T1, n = 13; CR post-4T1, n = 13; CR pre-4T1, n = 11. d AL, n = 11; FMD post-4T1, n = 12; LCC post-4T1, n = 12; CR post-4T1, n = 12; CR pre-4T1, n = 13 mice per treatment group. Source data are provided as a Source Data file.