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. 2021 Oct 27;11:21206. doi: 10.1038/s41598-021-00483-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison between label-free and TMT quantification methods. (A) Venn diagram showing the comparison of differentially upregulated proteins between ACPs in both datasets. (B) Venn diagram showing the comparison of differentially upregulated proteins between PCPs in both datasets. (C) Correlation analysis of fold changes in the label-free and TMT quantification data. (D) Validation of the proteomic data using protein expression. Western blotting was performed to measure EPCAM, P4HB and β-actin.