Figure 1.

Key steps of lymph node processing

(A and B) Two different representative lymph nodes in a mortar (A) or on a saline-soaked compress (B) immediately after excision. B) Lymph node or excessive fat tissue is highlighted with blue or green arrows, respectively.

(C) Lymph node in cell culture medium after being cut into small pieces.

(D) Lymph node pieces in a cell strainer. Tube filled with approximately 50 mL medium containing lymph node-derived cells.

(E) Pellet of lymph node-derived cells after centrifugation. Reddish stain indicates slight contamination with erythrocytes.