Cell culture medium

Reagent	Final concentration	Amount
RPMI (1X)	n/a	445 mL
Fetal bovine serum (FBS)	10%	50 mL
Penicillin 10,000 units/mL Streptomycin 10 mg/mL	100 units/mL 0.1 mg/mL	5 mL
Total	n/a	500 mL

Store at 4°C for three months, prepare and use under sterile conditions.