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. 2021 Oct 21;2(4):100914. doi: 10.1016/j.xpro.2021.100914

Washing buffer

Reagent Final concentration Amount
Phosphate-buffered saline (PBS) 1× n/a 492.5 mL
Fetal bovine serum (FBS) 1% 5 mL
EDTA 0.5% 2.5 mL
Total n/a 500 mL

Store at 4°C for three months.