Freezing medium
| Reagent | Final concentration | Amount |
|---|---|---|
| Fetal bovine serum (FBS) | 80% | 40 mL |
| Dimethyl sulfoxide (DMSO) | 20% | 10 mL |
| Total | n/a | 50 mL |
Store at 4°C for three months, prepare and use under sterile conditions.
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