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. 2021 Oct 21;2(4):100914. doi: 10.1016/j.xpro.2021.100914
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

FITC anti-human CD8 BioLegend RRID:AB_314110
BV421 anti-human CD19 BioLegend RRID:AB_11142678
BV605 anti-human CD20 BioLegend RRID:AB_2563398
PE anti-human Ig light chain κ BioLegend RRID:AB_493613
PE/Dazzle 597 anti-human Ig light chain λ BioLegend RRID:AB_2687261
PerCP/Cy5.5 anti-human CD3 BioLegend RRID:AB_2561628
PE/Cy7 anti-human CD5 BioLegend RRID:AB_2275812
AF700 anti-human CD4 BioLegend RRID:AB_493743
Viability Dye eFluor506 Thermo Fisher Scientific 65-0866-18

Chemicals, peptides, and recombinant proteins

Fetal bovine serum (FBS) Gibco 10500-064
Penicillin/Streptomycin Gibco 15140-122
EDTA Sigma Aldrich E8008
RPMI medium Gibco 21875-034
Trypan blue solution 0.4% Gibco 11538886
DMSO Serva 20385.01

Critical commercial assays

MS Columns Miltenyi 130-042-201
Dead Cell Removal Kit Miltenyi 130-090-101

Software and algorithms

FlowJo BD Biosciences RRID:SCR_008520
FACSDiva BD Biosciences RRID:SCR_001456

Other

Sterilizable forceps n/a n/a
Sterilizable scissors n/a n/a
Sterilizable mortar n/a n/a
Strainer 100 μm Greiner Bio-One 542000
Strainer 70 μm Greiner Bio-One 542070
Pasteur pipette LP Italiana 135030
Centrifuge Thermo Scientific Heraeus Megafuge 16R
Cryo vials Greiner Bio-One 122263
MACS Multistand Miltenyi 130-042-303
OctoMACS Separator Miltenyi 130-042-108