REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
FITC anti-human CD8 | BioLegend | RRID:AB_314110 |
BV421 anti-human CD19 | BioLegend | RRID:AB_11142678 |
BV605 anti-human CD20 | BioLegend | RRID:AB_2563398 |
PE anti-human Ig light chain κ | BioLegend | RRID:AB_493613 |
PE/Dazzle 597 anti-human Ig light chain λ | BioLegend | RRID:AB_2687261 |
PerCP/Cy5.5 anti-human CD3 | BioLegend | RRID:AB_2561628 |
PE/Cy7 anti-human CD5 | BioLegend | RRID:AB_2275812 |
AF700 anti-human CD4 | BioLegend | RRID:AB_493743 |
Viability Dye eFluor506 | Thermo Fisher Scientific | 65-0866-18 |
Chemicals, peptides, and recombinant proteins | ||
Fetal bovine serum (FBS) | Gibco | 10500-064 |
Penicillin/Streptomycin | Gibco | 15140-122 |
EDTA | Sigma Aldrich | E8008 |
RPMI medium | Gibco | 21875-034 |
Trypan blue solution 0.4% | Gibco | 11538886 |
DMSO | Serva | 20385.01 |
Critical commercial assays | ||
MS Columns | Miltenyi | 130-042-201 |
Dead Cell Removal Kit | Miltenyi | 130-090-101 |
Software and algorithms | ||
FlowJo | BD Biosciences | RRID:SCR_008520 |
FACSDiva | BD Biosciences | RRID:SCR_001456 |
Other | ||
Sterilizable forceps | n/a | n/a |
Sterilizable scissors | n/a | n/a |
Sterilizable mortar | n/a | n/a |
Strainer 100 μm | Greiner Bio-One | 542000 |
Strainer 70 μm | Greiner Bio-One | 542070 |
Pasteur pipette | LP Italiana | 135030 |
Centrifuge | Thermo Scientific | Heraeus Megafuge 16R |
Cryo vials | Greiner Bio-One | 122263 |
MACS Multistand | Miltenyi | 130-042-303 |
OctoMACS Separator | Miltenyi | 130-042-108 |