Table 2.

Seeding Volumes and Numbers for thawed T cells

Culture plate	Surface area	Volume	Cell number / well
48-well	1.1 cm2	0.55–0.6 mL	2.4 × 106
24-well	1.9 cm2	1.0–1.2 mL	4.0 × 106
12-well	3.5 cm2	2.1–2.2 mL	8.4 × 106
6-well	9.6 cm2	5.0–5.1 mL	20.0 × 106