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. 2021 Oct 27;12(11):1010. doi: 10.1038/s41419-021-04204-7

Fig. 3. Liver resident macrophages are responsible for protective effects of hepatocyte-derived EVs.

Fig. 3

A F4/80 (green) and DAPI (blue) staining of liver sections of the mice treated with PBS or PKH-26 labeled HepG2-EVs (red). B Timeline for the experiment of CL and EV treatments for CCL4-induced acute liver injury in mouse model. C HE staining of liver sections of the mice treated with CL + CCL4 + PBS or CL + CCL4 + HepG2-EVs. D The Serum GOT and GTP were measured for the mice treated with CL + CCL4 + PBS or CL + CCL4 + HepG2-EVs. E TUNEL staining of liver sections of the mice treated with CL + CCL4 + PBS or CL + CCL4 + HepG2-EVs. TUNEL positive cells were enumerated in 10 fields from 3 mice. Ratio to total cells was calculated. Statistical analysis was performed using student’s t test. Black and white bars indicate 50 μm. CL clodronate liposome.