Complete MegaGate LR Reaction
| Reagent | Amount |
|---|---|
| LR Clonase II Enzyme Mix | 1 uL |
| I-SceI (5 U/ul) | 1 uL |
| I-CeuI (5U/ul) | 1 uL |
| 10× CutSmart Buffer | 5 uL |
| pENTR vector | 50ng |
| pMegaDestination vector | 75ng |
| ddH2O | Fill to 50 uL |
| Total | 50 uL |
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