Table 2.

Summary of potency profiles of all PYY analogues lipidated from the N-terminal to position 32. In all analogues the fatty diacid (FA) moiety C18 diacid-γGlu-2xAdo was attached to the side chain of a lysine residue (analogues 2-30 and 52) or to the N-terminus (peptide 1). pEC₅₀ is calculated as the mean of at least three independent experiments, and the 95% confidence interval (CI) is given as [lower; upper]. The mean EC₅₀ in nM is calculated from the mean pEC50. For analogue 9 and 20 (data marked with *) two independent experiments were recorded.

No	FA	Y2 receptor		Y1 receptor		Y4 receptor
	Position	EC50 (nM)	pEC50 [95% CI]	EC50 (nM)	pEC50 [95% CI]	EC50 (nM)	pEC50 [95% CI]
PYY3-36	none	0.60	9.2 [9.2:9.1]	7.9	8.1 [8.2:7.9]	320	6.5 [6.7;6.4]
1	Nα	10	8.0 [8.3;7.8]	25	7.6 [7.9;7.3]	250	6.6 [6.6;6.5]
2	4	25	7.6 [8.1;7.6]	200	6.7 [7.0;6.7]	630	6.2 [6.5;6.4]
3	5	25	7.6 [7.8;7.4]	100	7.0 [7.3;6.7]	400	6.4 [6.5;6.2]
4	6	7.9	8.1 [8.3;7.9]	50	7.3 [7.5;7.1]	320	6.5 [6.7;6.3]
5	7	2.0	8.7 [9.0;8.3]	63	7.2 [7.2;7.1]	250	6.6 [7.3;5.9]
6	8	40	7.4 [7.6;7.2]	250	6.6 [7.0;6.3]	790	6.1 [6.2;5.9]
7	9	13	7.9 [8.2;7.6]	200	6.7 [6.9;6.5]	630	6.2 [6.4;6.1]
8	10	1.3	8.9 [9.3;8.6]	50	7.3 [7.6;7.0]	500	6.3 [6.6;6.0]
9	11	5.0	8.3 [8.6;8.1]	50*	7.3*[-.-;-.-*]	> 1000	<6.0
10	12	16	7.8 [7.8;7.7]	200	6.7 [6.8;6.5]	500	6.3 [6.4;6.1]
11	13	32	7.5 [7.8;7.2]	320	6.5 [6.7;6.4]	500	6.3 [6.5;6.0]
12	14	4.0	8.4 [8.8;9.9]	63	7.2 [7.5;6.8]	1000	6.0 [6.2;5.9]
13	15	10	8.0 [8.3;7.8]	63	7.2 [7.3;7.1]	1000	6.0 [6.1;6.0]
14	16	20	7.7 [8.2;7.3]	160	6.8 [7.3;6.2]	> 1000	6.0
15	17	5.0	8.3 [8.9;7.6]	100	7.0 [7.5;6.5]	500	6.3 [7.1;5.5]
16	18	7.9	8.1 [8.3;7.9]	63	7.2 [7.5;7.0]	400	6.4 [6.6;6.1]
17	19	4.0	8.4 [8.8;8.0]	63	7.2 [7.4;7.0]	500	6.3 [7.1;5.6]
18	20	20	7.7 [8.0;7.4]	790	6.1 [6.2;6.0]	> 1000	<6.0
19	21	4.0	8.4 [8.7;8.0]	130	6.9 [7.2;6.7]	> 1000	<6.0
20	22	2.0*	8,7*[-.-;-.-*]	40	7.4 [7.4;7,3]	500*	6.3*[−.−; −.−*]
21	23	13	7.9 [8.4;7.5]	63	7.2 [7.4;7.0]	400	6.4 [6.5;6.3]
22	24	50	7.3 [7.6;7.0]	400	6.4 [6.6;6.3]	790	6.1 [6.4;5.8]
23	25	79	7.1 [8.0;6.1]	500	6.3 [6.9;5.7]	630	6.2 [6.6;5.7]
24	26	40	7.4 [7.5;7.3]	320	6.5 [6.5;6.4]	500	6.3 [6.6;6.0]
25	27	32	7.5 [8.0;6.9]	320	6.5 [6.8;6.2]	400	6.4 [6.6;6.3]
26	28	32	7.5 [7.7;7.3]	1000	6.0 [6.2;5.9]	>1000	<6.0
27	29	500	6.3 [6.8;5.8]	500	6.3 [7.0;5.6]	790	6.1 [6.5;5.8]
28	30	5.0	8.3 [8.5;8.2]	630	6.2 [6.4;5.9]	79	7.1 [7.3;6.9]
29	31	79	7.1 [7.2;7.1]	320	6.5 [6.6;6.4]	630	6.2 [6.4;6.1]
30	32	50	7.3 [7.4;7.2]	>1000	<6.0	>1000	<6.0
52	30	4.0	8.4 [8.6;8.3]	500	6.3 [6.5;6.0]	40	7.4 [7.4;7.3]