Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 27;11:21227. doi: 10.1038/s41598-021-00691-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The effects of Mx2 depletion on antiviral innate response and intrinsic mRNA accumulation of innate immune genes in resistant A172 cells. (A) Investigation of nuclear IRF3 in A172 cells via immunofluorescence staining. (B) Quantification of IRF3 localized in the A172 nucleus by automatic counting using Image J software. 10–15 fields of each slide were randomly chosen for counting the numbers of IRF3-DAPI coincident dots. (C) ELISA quantification of secreted IFNα and IFNβ in cell supernatant. RQ: relative quantity, values that correspond to optical densities at OD 450 nm compared to values in NC siRNA-transfected A172 cells. (*p < 0.05; **p < 0.01; compared with NC siRNA-treated A172 cells. Error bars represent SD.) (D) Expression of effector proteins associated with the NF-κB signaling pathway. Blots were cut and probed with indicated antibodies, respectively. β-actin serves as a loading control. (E) mRNA accumulation of indicated immune genes in Mx2-depleted A172 cells. Mx2-depleted or mock-depleted A172 cells were harvested at 24 h post-transfection. Total RNA was isolated and analyzed by qPCR assay for mRNA levels of indicated immune genes. Used primers are shown in Table 3. RQ: relative quantity, compared to transcript levels in mock-depleted A172 cells. Bar, mean ± SD. GAPDH serves as a normalization control.