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. 2021 Oct 27;11:21222. doi: 10.1038/s41598-021-00743-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Conjugation levels for Lys sites of Her-IgG (100 μg) (left) generated by reaction with a: NHS-PEG-N₃ (7.71 mM) for 1 h, b: AD-PEG-N₃ (0.30 mM) for 1 h, c: AD-PEG-N₃ (3.0 mM) for 1 h, d: AD-PEG-N₃ (2.89 mM) for 4 h with gradual addition, and e: formaldehyde (0.57 mM) for 1 h. The L/P (~ 4) and L/K_M ratios are indicated in the bottom of the map. The deconvoluted spectra of the light chain (right top) and heavy chain (right bottom) of “d” and “a” shown in the left.