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. 2021 Oct 27;12(11):1008. doi: 10.1038/s41419-021-04319-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a IFC co-staining of TH and NeuN, and IFC staining of NeuN in the ventral midbrain sections from 6 and 15-month-old Pitx3^cWT and Pitx3^cKO mice. SNc and VTA were outlined, respectively (scale bar: 10 μm). b Quantification of TH⁺/NeuN⁺ and NeuN+ neurons in the SNc and VTA from 6- and 15-months-old Pitx3^cWT and Pitx3^cKO mice (N = 3 mice per genotype). unpaired t-test, ***p = 0.0008 (15 months old for TH⁺/NeuN⁺ co-staining); unpaired t-test, *p = 0.0424 (15 months old for NeuN⁺ staining). c IFC staining of cleaved-caspase3 in the SNc area from 6 and 15-month-old Pitx3^cWT and Pitx3^cKO mice (scale bar: 10 μm). d Quantification of the percentage of neurons with cleaved-caspase3⁺ puncta in SNc (N = 3 mice per genotype). unpaired t-test, **p = 0.0062.