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. 2021 Oct 14;12:754316. doi: 10.3389/fimmu.2021.754316

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Copyright © 2021 Scheurer, Kitt, Huber, Fundel-Clemens, Pflanz, Debatin and Strauss

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro-generated MDSCs consist of CD11b+CD11c+ and CD11b+CD11c− MDSCs. MDSCs were generated from BM cells in the presence of GM-CSF. (A) After 4 days, MDSCs were stained for CD11b and Gr-1 expression or (B) for CD11b and CD11c expression. (C) Expression of Gr-1 was determined on CD11b+CD11c+ and CD11b+CD11c− MDSCs, and CD11b+CD11c+ and CD11b+ and CD11c+ MDSCs were designated to M-MDSCs or PMN-MDSCs by staining Ly-6C and Ly-6G. (D) Staining of various surface markers to define differences in the expression on CD11b+CD11c+ and CD11b+CD11c− MDSCs. FACS diagrams show one representative experiment out of at least three experiments performed.