TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli XL2 blue | Stratagene | |
| S. suis | ||
| 10 | Virulent serotype 2 strain | 32 |
| 3 | Serotype 2 | 28 |
| 735 | Reference serotype 2 strain | 28 |
| 6555 | Reference serotype 1 strain | 28 |
| 6388 | Serotype 1 | 28 |
| 6290 | Serotype 1 | 28 |
| 5673 | Serotype 1/2 | 28 |
| 5679 | Serotype 1/2 | 28 |
| 5209 | Reference serotype 1/2 strain | 28 |
| 5218 | Reference serotype 9 strain | 28 |
| 5973 | Serotype 9 | 28 |
| 6437 | Serotype 9 | 28 |
| Reference strains | Serotypes 1 to 34 | 7, 8, 10 |
| Plasmids | ||
| pKUN19 | Replication functions of pUC, Ampra | 17 |
| pCPS1-1 | pKUN19 containing 5-kb EcoRV fragment of cps operon of type 1 | This work (Fig. 1B) |
| pCPS1-2 | pKUN19 containing 2.2-kb HindIII fragment of cps operon of type 1 | This work (Fig. 1B) |
| pCPS9-1 | pKUN19 containing 1-kb HindIII-XbaI fragment of cps operon of serotype 9 | This work (Fig. 1C) |
| pCPS9-2 | pKUN19 containing 4.0-kb XbaI-XbaI fragment of cps operon of serotype 9 | This work (Fig. 1C) |
Ampr, ampicillin resistant.