TABLE 1.
Bacterial strains and plasmids
Strain or plasmid | Relevant characteristic(s) | Source or reference |
---|---|---|
Strains | ||
E. coli XL2 blue | Stratagene | |
S. suis | ||
10 | Virulent serotype 2 strain | 32 |
3 | Serotype 2 | 28 |
735 | Reference serotype 2 strain | 28 |
6555 | Reference serotype 1 strain | 28 |
6388 | Serotype 1 | 28 |
6290 | Serotype 1 | 28 |
5673 | Serotype 1/2 | 28 |
5679 | Serotype 1/2 | 28 |
5209 | Reference serotype 1/2 strain | 28 |
5218 | Reference serotype 9 strain | 28 |
5973 | Serotype 9 | 28 |
6437 | Serotype 9 | 28 |
Reference strains | Serotypes 1 to 34 | 7, 8, 10 |
Plasmids | ||
pKUN19 | Replication functions of pUC, Ampra | 17 |
pCPS1-1 | pKUN19 containing 5-kb EcoRV fragment of cps operon of type 1 | This work (Fig. 1B) |
pCPS1-2 | pKUN19 containing 2.2-kb HindIII fragment of cps operon of type 1 | This work (Fig. 1B) |
pCPS9-1 | pKUN19 containing 1-kb HindIII-XbaI fragment of cps operon of serotype 9 | This work (Fig. 1C) |
pCPS9-2 | pKUN19 containing 4.0-kb XbaI-XbaI fragment of cps operon of serotype 9 | This work (Fig. 1C) |
Ampr, ampicillin resistant.