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. 2021 Oct 14;11:733848. doi: 10.3389/fonc.2021.733848

Figure 3.

Figure 3

CK1α sustains NF-kB signaling pathway. Representative WB of NF-ĸB dependent signaling in MCL cell lines Jeko-1, Granta-519 and Rec-1, treated with D4476 40uM for 48h (A), in Jeko-1 IPTG inducible CSNK1A1 directed shRNA clone (named CK1α shRNA) and in Jeko-1 wt treated with IPTG 500µM for 13 days (B left panel) or Jeko -1 cells electroporated with CSNK1A1 directed siRNAs (B right panel), or in Granta-519 IPTG inducible CSNK1A1 directed shRNA clone (named CK1α shRNA) treated with IPTG 500µM for 72h (C). Membranes for WB analysis were probed with antibodies listed in the figure. GAPDH or β actin were used as loading control. Experiments were repeated at least three times (D). BIRC3 mRNA expression in Jeko-1 treated with D4476 40uM for 48h, (left panel) or in Jeko-1 IPTG inducible CSNK1A1 directed shRNA clone treated with IPTG 500µM for 13 days (middle panel) or in Granta-519 IPTG inducible CSNK1A1 directed shRNA clone treated with IPTG 500µM for 7 days (right panel). Data represent mean ± SD of at least 3 independent experiments. * indicates p < 0.05.