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. 2021 Oct 14;11:733848. doi: 10.3389/fonc.2021.733848

Figure 4.

Figure 4

CK1α associates with the CBM1 complex, and its silencing reduces basal and anti-IgM induced NF-kB activation. (A) Immunoprecipitation (IP) of CARD11 and CK1α in Jeko-1 (A), Granta- 519 (B), purified B lymphocytes from two MCL patients (pt#1 and pt#2) (C), stimulated with anti-IgM 10µg/ml for 5 min (serum= preimmune serum). (D) CK1α silencing reduces the binding of CK1α to CARD11 and BCL10 in the CBM1 complex in anti-IgM stimulated Jeko-CK1α shRNA inducible clone. Untreated and IPTG treated (500µM for 72h) Jeko-1 cells from the CK1α shRNA cellular clone were stimulated with anti-IgM 10µg/ml for 5 min and IP was carried out using CARD11 antibody. (E) CK1α silencing affects basal and anti-IgM stimulated NF-κB activation. Cells were treated as in (D). WB was probed with antibodies listed in the figure. β-actin was used as loading control.

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