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. 2021 Oct 8;2(4):100859. doi: 10.1016/j.xpro.2021.100859
REGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
RPMI 1640 Medium Life Technologies 11875-119
B27 supplements Life Technologies 17504-044
Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free Corning 356231
DMEM/F12 Gibco/Life Technologies 12634028
Accutase Sigma-Aldrich A6964
KnockOut Serum Replacement Thermo Fisher Scientific 10828-028
Pluronic™ F-127 (20% Solution in DMSO) Thermo Fisher Scientific P3000MP
Fura-2, AM, cell permeant Thermo Fisher Scientific F1221
DPBS, no calcium, no magnesium Fisher Scientific 14-190-250
NaCl Sigma-Aldrich S9888
KCl Sigma-Aldrich 529552
CaCl2 1 M in H2O Sigma-Aldrich 21115
Glucose Sigma-Aldrich G8270
MgCl2 Sigma-Aldrich M8266
HEPES Sigma-Aldrich H3375
NaOH 10 M in H2O Sigma-Aldrich 72068
Trypan blue stain Life Technologies T10282
Experimental models: Cell lines
Human induced pluripotent stem cell lines Stanford Cardiovascular Institute Biobank N/A
Bacterial and virus strains
ASAP2 lentivirus ASAP2 plasmid is from Michael Lin’s Lab N/A
Other
Polystyrene Storage Bottle, 250 mL VWR 8390
6-Well culture plate E & K Scientific EK-27160
96-Well flat clear bottom black polystyrene TC-treated microplates Corning 3603
35 mm Glass bottom dish with 20 mm micro-well #1.5 cover glass Cellvis D35-20-1.5-N
Rectangular glass coverslips Warner Instruments 64-0709
100 mm × 20 mm Petri dish, sterile Sigma-Aldrich P5606
Dow Corning High Vacuum Grease Sigma-Aldrich Z273554-1EA
Fisherbrand Razor Blades Fisher Scientific 12-640
Nikon Immersion Oil (Type F) 30cc PFS Morrell Instrument Company MXA22168
Conical tubes, 15 mL Corning Falcon 352097
LoBind micro-centrifuge tubes Fisher Scientific 13-698-794
5 mL Pipettes sterile, individually wrapped Fisher Basix 14-955-232
10 mL Pipettes sterile, individually wrapped Fisher Basix 14-955-234
2 mL Aspiration pipettes sterile, individually wrapped Corning Falcon 357558
Reservoir, 10 mL sterile MTC Bio P8010-5S
Tube micro centrifuge, 0.5 mL Dynalon Labware 299265
Nalgene™ Rapid-Flow™ Sterile Single Use Vacuum Filter Units Fisher Scientific 09-741-04
Paint brush Amazon N/A
Narrow bath chamber with field stimulation Warner Instruments RC-27NE2
Magnetic heated platforms Warner Instruments PM-6
Class II, type A2 or better biosafety cabinet Thermo Fisher Scientific 1300 Series Class II
CO2 laboratory incubator Laboratory Equipment Company MCO-170AICUVL-PA
Centrifuge VWR Centrifuge 5810 R
pH meter Hanna Instruments HI2002-01
Lab companion stirring hot plate, ceramic top Cole-Parmer 8657808
Magnetic stirring bars Fisher Scientific 14-513-68
Aspirator vacuum N/A N/A
Countess® Cell Counting Chamber Slides Thermo Fisher Scientific C10312
Luna-FL automated fluorescence cell counter WISBIOMED LB-L20001
Sony SI8000 Cell Motion Imaging System Sony Biotechnology N/A
Lambda DG 4 Sutter Instrument Company N/A
Zeiss LSM 710 confocal microscope Zeiss N/A
Software and algorithms
GraphPad Prism version 7.0 GraphPad Software N/A
ImageJ NIH N/A
Sony SI8000 cardiac analysis software Sony Biotechnology N/A