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. 2021 Oct 14;8:757256. doi: 10.3389/fnut.2021.757256

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Copyright © 2021 Teixeira, Torrez Lamberti, DeBose-Scarlett, Bahadiroglu, Garrett, Gardner, Meyer, Lorca and Gonzalez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SREBP1 and SCAP protein levels determined via western blot. β-actin levels were used as a loading control. (A,B) Ratios of the inactive (122 kDa) and active (65 kDa) forms of SREBP1 over β-actin (42 kDa) band intensity, respectively. (C) Ratio of SCAP over actin band intensity and (D) over the active form of SREBP1. (E) Representation of the band intensity of SREBP1, SCAP, and β-actin from each treatment group. n = 4 from 3 independent experiments. HF, high-fat diet; LC, low-calorie diet; BB, blueberry extract; LJ, L. johnsonii N6.2; BB/LJ, blueberry extract + L. johnsonii N6.2. Blue bars: animals under HF diet; orange bars: animals under LC diet. *p-value < 0.05; **p-value < 0.01.