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. 2021 Oct 16;2(4):100886. doi: 10.1016/j.xpro.2021.100886

Figure 1.

Figure 1

The cloning strategy of full-length HTT cDNA in a modified pFastBac1 vector

(A) A new linker cassette was inserted in pFastBac1 (Thermo Fisher Scientific, 4775 bp) vector. The linker cassette contains a FLAG tag, a 6×His-tag, a TEV protease recognition site and restriction enzyme sites (NcoI, XhoI, SacII and others, underlined and shown in italic).

(B) Htt cDNA fragment containing huntingtin amino acid 1–171 was inserted into the modified pFastBac1 vector with NcoI and XhoI restriction sites and then the rest of Htt cDNA fragment containing huntingtin amino acid 172-3,144 was cloned into the vector with XhoI and SacII restriction sites to express HTT tagged with FLAG, HIS and TEV sites. Each Htt DNA fragments and vector size are not up to scale.