Table 1.
The detailed PCR composition
| Component | Amount (μl) |
|---|---|
| Bacmid DNA (final 100 ng) | 1 |
| 2×TOPsimple™ PreMIX-Tenuto | 25 |
| 5×Band Doctor™ | 10 |
| M13 forward primer (final 10 pmol) | 1 |
| hHTT R146 reverse primer (final 10 pmol) | 1 |
| Sterile Water | 12 |
| Total | 50 |
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