Table 2.

The reaction conditions of the PCR reaction for the bacmid plasmid containing HTT gene

PCR cycling conditions
Steps	Temperature	Time	Cycles
Initial Denaturation	94°C	3 min	1
Denaturation	94°C	45 s	30 cycles
Annealing	55°C	45 s
Extension	72°C	3+α min a
Final extension	72°C	7 min	1
Hold	4°C	-

^aDepending on the number of CAG repeats, add extra min for extension (α=0.18 s per one CAG repeat).