Reagent	Final concentration	Amount
Tryptone	1% (w/v)	5 g
Yeast extract	0.5% (w/v)	2.5 g
NaCl	1% (w/v)	5 g
Agar	1.5% (w/v)	7.5 g
Sterile Water	N/A	Up to 500 mL
Total	N/A	500 mL
Kanamycin (50 mg/mL)	50 μg/mL	500 μL
Gentamycin (50 mg/mL)	7 μg/mL	70 μL
Tetracycline (5 mg/mL)	10 μg/mL	1 mL
Bluo-Gal (200 mg/mL)	100 μg/mL	250 μL
IPTG (119 mg/mL)	40 μg/mL	168 μL