Reagent | Final concentration | Amount |
---|---|---|
5 M NaCl | 150 mM | 1.5 mL |
1 M Tris-HCl (pH8.0) | 50 mM | 2.5 mL |
100% Glycerol | 5% (v/v) | 2.5 mL |
Sterile Water | N/A | 43.5 mL |
Total | N/A | 50 mL |
FLAG peptides | 0.4 mg/mL | N/A |
Reagent | Final concentration | Amount |
---|---|---|
5 M NaCl | 150 mM | 1.5 mL |
1 M Tris-HCl (pH8.0) | 50 mM | 2.5 mL |
100% Glycerol | 5% (v/v) | 2.5 mL |
Sterile Water | N/A | 43.5 mL |
Total | N/A | 50 mL |
FLAG peptides | 0.4 mg/mL | N/A |