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. 2021 Oct 13;7(10):e08171. doi: 10.1016/j.heliyon.2021.e08171

Figure 8.

Figure 8

I; Photomicrographs of Tumor necrosis factor-α (TNF-α) (I; A-C) and caspase 3 (II; D-F) immuno-stained lung sections of different groups. II; The percentage area of TNF-α (IIa) and caspase 3 (IIb)in all experimental groups. I (A-C): Photomicrographs of Tumor necrosis factor-α (TNF-α) immuno-stained lung sections of different groups: lung sections of the control group (A) show a negative immune reaction. The lung section of BUS group (B) shows markedly increased immuno-positive reaction in the pulmonary interstitium (asterisk) and mononuclear cells (white arrowheads). The lung section of BUS + ALA group IV (C) shows the mild immuno-positive reaction in the pulmonary interstitium (black arrowheads) (TNF-α immune stain x 400). I (D-F): Photomicrographs of caspase 3 immuno-stained lung sections of different groups: lung sections of the control group (A) show a negative immune reaction. The lung section of BUS group (B) shows markedly increased immuno-positive reaction in the pulmonary interstitium (asterisks) and mononuclear cells (white arrowheads). The lung section of the BUS + ALA group (C) shows the mild immuno-positive reaction in mononuclear cells (black arrowhead) (caspase 3 immune stain x 400). II: The percentage area of TNF-α (IIa) and caspase (IIb) in all experimental groups. The test used: One-way ANOVA followed by posthoc Tukey's test. Data are expressed as mean ± SD, # represents significance compared with the control group; ∗ represents significance compared with the BUS group.