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. 2021 Oct 14;12:761249. doi: 10.3389/fendo.2021.761249

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Copyright © 2021 Lei, Zhang, van Pelt and Hamer

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromosome synapsis was completed without the addition of fresh mGSCs. (A, B) In vitro - generated pachytene spermatocytes stained for SYCP3 (red), MDC1 (white), (A) HORMAD1 (green) or (B) SYCP1 (green) were observed both group A (undifferentiated mGSCs on a fresh layer of proliferatively inactivated SK49 Sertoli cells) and group B (a fresh layer of proliferatively inactivated SK49 Sertoli cells alone) from day 8 to day 12. (C) In vitro- generated pachytene spermatocytes stained for SYCP1 (red), MLH1 (green) and MDC1 (white) in group (A, B). Scale bars, 5μm.